MiR-28-3p regulates high glucose-induced endothelial dysfunction by targeting CXXC5.

ABSTRACT:

Introduction

Data from the CEO database show that microRNA (miR)-28-3p levels are elevated in diabetic patients. Nevertheless, the role of miR-28-3p in peripheral artery disease with diabetes has not been investigated.

Material and methods

The levels of miR-28-3p, CXXC5, CXXC-type zinc finger protein 5, and CXXC5's downstream molecules as well as endothelial function were investigated. Dual luciferase analyses were used to confirm the binding site of miR-28-3p and CXXC5.

Results

Under high-glucose conditions, miR-28-3p expression is upregulated, whereas CXXC5 expression is downregulated. Overexpression of miR-28-3p increased cell apoptosis and inhibited cell proliferation, migration, and vessel formation, whilst inhibiting its expression had the opposite effect. The o

SUBMITTER: Wang L 

PROVIDER: S-EPMC12305552 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature