Protocol for GCaMP expression and in vivo calcium imaging of medial prefrontal cortex neurons in freely behaving HIV-1 Tat transgenic mice.

ABSTRACT: Advancements in imaging techniques have enhanced our ability to study brain structure and function. Genetically encoded calcium indicators enable high-resolution visualization of neuronal activity at the cellular level. Here, we present a protocol for imaging medial prefrontal cortex (mPFC) neurons in a freely behaving neuroHIV mouse model. We describe steps for survival surgery preparation, craniotomy, durotomy, and adeno-associated virus (AAV) injection. We then detail procedures for implanting the gradient-refractive-index (GRIN) lens, building a headcap, and checking viral expression. For complete details on the use and execution of this protocol, please refer to Yadav-Samudrala et al.¹.

SUBMITTER: Yadav-Samudrala BJ 

PROVIDER: S-EPMC12305568 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature