Isolation of Verrucosins A-E from a Marine <i>Verrucosispora</i> sp. Reveals a Unifying Biosynthetic Hypothesis for Linear and Macrocyclic Polyketides.

ABSTRACT: As part of our long-standing program evaluating the biosynthetic complexity and biomedical potential of natural products from marine microbes, our attention was drawn to culture extracts from a Verrucosispora sp. (strain TAA-831), which produced multiple compounds with unique UV absorbance signatures and HRMS data. Large-scale fermentation and targeted isolation afforded verrucosins A-E (1-5), a mixture of linear and macrocyclic polyketides whose structures were determined through a synergistic combination of experimental, computational, and genomic approaches. The conserved sequence of methyl malonate and malonate motifs across the verrucosins implied a shared biosynthetic origin despite structural divergence as linear and cyclic congeners. Targeted genome mining reve

SUBMITTER: Holland DC 

PROVIDER: S-EPMC12305660 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature