Functional investigation of the RNA helicase MOV10 with respect to its interplay with factors involved in nonsense-mediated mRNA decay.

ABSTRACT: The RNA helicase Moloney leukemia virus 10 (MOV10) is involved in several RNA processing pathways, including RNA silencing, defense against viral RNA and nonsense-mediated mRNA decay (NMD). MOV10 is a member of the Up-frameshift 1 (UPF1)-family of superfamily 1 (SF1) helicases and like its prototype member, unwinds RNA duplexes bearing a 5'-single-stranded overhang. Sequence comparisons of MOV10 and UPF1 revealed significant identity between their RecA domains and considerable divergence between the N-terminal domains preceding the helicase core. Using in vitro biochemical approaches, we show that the N-terminal domain of MOV10 is functionally distinct from the CH domain of UPF1, both in terms of its impact on catalytic activity and the protein-protein interactions it mediates. MOV10 engag

SUBMITTER: Xue G 

PROVIDER: S-EPMC12305709 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature