Recombinant tritin protein exhibits antiviral activity against zucchini yellow mosaic virus.

ABSTRACT:

Background

Ribosome-inactivating proteins (RIPs) are a group of proteins known to inhibit protein synthesis and contribute to plant defense responses. Although the antiviral properties of various RIPs have been demonstrated, the antiviral potential of tritin, a type 1 RIP from bread wheat (Triticum aestivum L.), remains unexplored. This study aimed to investigate the antiviral activity of recombinant tritin against zucchini yellow mosaic virus (ZYMV) in zucchini (Cucurbita pepo L.) plants.

Methods

The tritin gene was isolated from the wheat cultivar Kutluk-94, cloned into the pETDuet-1 expression vector, and expressed in Escherichia coli BL21 (DE3) cells. Following induction, recombinant tritin was purified using Ni-NTA affinity chromatography. Antiviral activity was assesse

SUBMITTER: Demirel S 

PROVIDER: S-EPMC12305900 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature