Design of genus-specific semi-nested primers for simple and accurate identification of Enterobacter strains.

ABSTRACT:

Background

The genus Enterobacter, in the family Enterobacteriaceae, is of both clinical and environmental importance. This genus has undergone frequent taxonomic changes, making it challenging to identify taxa even at genus level. This study aimed to design Enterobacter genus-specific primers that can be used for simple PCR identification of large sets of putative Enterobacter isolates.

Results

Comparative genomic approaches were employed to identify genes that were universally present on Enterobacter genomes but absent from the genomes of other members of the family Enterobacteriaceae, based on an initial set of 89 genomes. The presence of these genes was further confirmed in 4,276 Enterobacter RefSeq genomes. While no strictly genus-specific genes were identified, the hpa

SUBMITTER: Jordan S 

PROVIDER: S-EPMC12305906 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature