Capture and detection of extracellular vesicles derived from human breast cancer cells using a 3D self-assembled nanostructured SiO₂ microfluidic chip.

ABSTRACT:

Background

Tumor-derived extracellular vesicles offer a minimally invasive approach to evaluate tumor progression and metastasis. However, detecting biomarkers, such as extracellular vesicles in body fluids during the early stages of disease, remains a significant challenge. Conventional methods like ultracentrifugation-based isolation or Western blot protein quantification are time-consuming, require large sample volumes, and offer low yield and sensitivity. Therefore, the development of new biosensors for the specific and efficient analysis of tumor extracellular vesicles is urgently needed.

Methods

Microfluidic devices provide extraordinary benefits for bioanalysis, offering a large surface area for the contact between target molecules and the biosensor, significantly enh

SUBMITTER: Cabeza C 

PROVIDER: S-EPMC12305923 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature