CLCF1 contributes to high-glucose-induced EndMT by modulating JAK2/STAT3 signaling.

ABSTRACT:

Background

Cardiotrophin-like cytokine factor 1 (CLCF1) is shown to enhance glomerular albumin permeability, contributing to proteinuria. However, its specific involvement in diabetic kidney disease (DKD) remains undefined. This study aimed to explore the mechanistic contribution of CLCF1 to endothelial-to-mesenchymal transition (EndMT) in DKD.

Methods

CLCF1 expression in patients with DKD and high glucose (HG)-stimulated human glomerular endothelial cells (HRGECs) was quantified via quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry (IHC). The effects of CLCF1 on HG-induced EndMT and the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling cascade were assessed through qRT-PCR and Western bl

SUBMITTER: Deng R 

PROVIDER: S-EPMC12305993 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature