Electrochemical Investigation of Enzyme Kinetics with an Unmediated, Unmodified Platinum Microelectrode: The Case of Glucose Oxidase.

ABSTRACT: We present a simple analytical method for studying the physical parameters governing redox-active enzyme kinetics using microscale electrodes. With the enzyme freely diffusing in solution, the interaction with its natural substrates produces a linearly increasing current corresponding to the reaction rate and the intrinsic thermodynamic properties of the enzyme. We show that external complications, such as artificial mediators or enzyme surface immobilization, can be avoided by using an unmediated, unmodified platinum microelectrode and that control over the dominating kinetic process can be readily achieved by changing the enzyme and (co)substrate concentrations. This is achieved using the glucose oxidase (GOx)/glucose system to compare with standard practice UV-Vis techniques, including

SUBMITTER: Davis C 

PROVIDER: S-EPMC12306184 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature