Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization.

ABSTRACT: The integration of DNA and protein-designed nanostructures represents a transformative approach to the development of programmable biopolymers for nanoscale construction. While DNA nanostructures excel in the readily programmable precision and scalability of base pairing, protein assemblies exploit the chemical diversity of amino acids for greater functional versatility. Here a platform is presented that unifies these two paradigms by combining coiled-coil protein origami with DNA nanostructures through orthogonal protein-protein (SpyCatcher-SpyTag) and protein-DNA (DCV-DNA) covalent conjugation strategies. This dual-functionalization strategy enables the construction of stable and versatile protein-DNA composites capable of hierarchical self-assembly. This shows that these composites driv

SUBMITTER: Zhou W 

PROVIDER: S-EPMC12306420 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature