Metaproteomics-based stable isotope fingerprinting links intestinal bacteria to their carbon source and captures diet-induced substrate switching.

ABSTRACT: Diet has strong impacts on the composition and function of the gut microbiota with implications for host health. Therefore, it is critical to identify the dietary components that support growth of specific microorganisms in vivo. We used protein-based stable isotope fingerprinting (Protein-SIF) to link microbial species in gut microbiota to their carbon sources by measuring each microorganism's natural 13C content (δ13C) and matching it to the 13C content of available substrates. We fed gnotobiotic mice, inoculated with a 13 member microbiota, diets in which the 13C content of all components was known. We varied the source of protein, fiber, or fat to observe 13C signature changes in microbial consumers of these substrates. We observed significant changes in the δ13C values and abundances

SUBMITTER: Mordant A 

PROVIDER: S-EPMC12306441 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature