Hypoxic niches established via endogenous oxygen production in scaffold under anoxia for enhanced bone regeneration.

ABSTRACT: Anoxia remains a challenging problem to effective graft implantation in bone tissue engineering for managing large-size bone defects. One promising strategy is to provide immediate oxygen required for cell viability and graft maturation by introducing oxygen-generating biomaterials. In this study, we present a novel composite oxygen-generating scaffold by integrating oxygen-generating microspheres (OMs) comprised of emulsified calcium peroxides (CPOs) encapsulated in poly (lactic-co-glycolic acid; PLGA) into the gelatin methacryloyl (GelMA) hydrogel. The in vitro results reveal that the scaffold encapsulating 2% (w/v) OMs (OM@GelMA) mildly sustained oxygen production for approximately 16 days, and hence, established hypoxic niches with low oxygen tension (10-46 mmHg) under anoxic cu

SUBMITTER: Gan K 

PROVIDER: S-EPMC12306443 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature