Engineering <i>Escherichia coli</i> Pyruvate Metabolism to Generate Noncanonical Reducing Power.

ABSTRACT: The future of biomanufacturing is dependent on rewiring biological systems to establish an alternative approach to our current chemical industries. However, a key limitation in biomanufacturing is that desired processes must rely on the same two redox cofactors as natural metabolism, nicotinamide adenine dinucleotide (phosphate) NAD(P)⁺, to shuttle electrons energy. Thus, competition of resources with natural reactions within host cells is nearly unavoidable. One strategy to overcome redox cofactor resource competition is the implementation of a third, noncanonical redox cofactor, such as nicotinamide mononucleotide (NMN⁺), which supports specific electron delivery to desired reactions. Here, we redesign the Escherichia coli pyruvate dehydrogenase multienzyme c

SUBMITTER: Aspacio D 

PROVIDER: S-EPMC12306648 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature