TRPV1 Defends the Healthy Murine Cornea Against Staphylococcus aureus Adhesion Independently of Sensory Nerve Firing.

ABSTRACT:

Purpose

Previously, we showed that transient receptor potential ion channels TRPA1 and TRPV1 selectively protect the cornea against bacterial adhesion, with TRPA1 countering the Gram-negative pathogen Pseudomonas aeruginosa (P. aeruginosa) and TRPV1 countering environmental bacteria. Here, we explored parameters of this specificity using a Gram-positive pathogen Staphylococcus aureus (S. aureus).

Methods

Healthy corneas of C57BL/6J wild-type (WT), TRPA1 (-/-), or TRPV1 (-/-) mice were challenged with S. aureus for 4 or 6 hours. Some experiments instead/also used resiniferatoxin (RTX) to deplete TRPV1-expressing nerves, JNJ-17203212 to selectively antagonize TRPV1, or the anesthetic bupivacaine to inhibit nerve firing. Adherent bacteria were quantified using fluorescence in s

SUBMITTER: Flandrin O 

PROVIDER: S-EPMC12306694 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature