Identification of differentially expressed genes associated with ferroptosis in ulcerative colitis.

ABSTRACT:

Purpose

To identify ferroptosis-related genes associated with the development of Ulcerative colitis (UC), through bioinformatics and basic experiments.

Methods

Ferroptosis-related genes were identified from UC microarray data extracted from the GEO database and FerrDb. GO and KEGG pathway enrichment analyses were performed. Hub genes were identified through PPI analysis, leading to TF-hub gene and miRNA-hub gene regulatory network, predicting potential drug candidates by DSigDB. RT-qPCR, WB and IHC were employed to validate hub gene expression in animal samples. Clinical samples were gathered from Normal examiners and UC patients and IHC was performed to verify SLC7A5.

Results

Eleven ferroptosis-related DEGs were identified (nine upregulated and two downregulated gene

SUBMITTER: Zhang F 

PROVIDER: S-EPMC12306769 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature