Oligosaccharides from the seeds of <i>Dolichos lablab L.</i> promote gut microbial metabolite L-arginine production to alleviate cyclophosphamide-induced immunosuppression.

ABSTRACT:

Introduction

Dolichos lablab L. is a nutritionally and medicinally significant legume, yet research on its bioactive oligosaccharides remains limited. This study investigates the potential of Dolichos lablab L. oligosaccharides to ameliorate cyclophosphamide (CTX)-induced immunosuppression and intestinal damage.

Methods

Crude oligosaccharides were purified to yield mixture SRSV (86% sugar content), comprising sucrose, raffinose, stachyose, and verbascose (mass ratio 19:16.8:50:14.2). Immunosuppressed mice (CTX-induced) were treated with SRSV (150 mg/kg). Gut microbiota (GM) diversity was analyzed via 16S rRNA sequencing, and serum metabolites were profiled using metabolomics. GM-depleted mice (antibiotic-treated) and L-arginine supplementation experiments were

SUBMITTER: Liu B 

PROVIDER: S-EPMC12307210 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature