Immune microenvironment heterogeneity characterizes biologically distinct KRAS^mut/SPOP^mut and KRAS^mut/PIK3CA^mut mesonephric-like adenocarcinoma subtypes revealed by integrated whole-exome and transcriptomic profiling.

ABSTRACT:

Objective

This study aims to uncover the molecular biology and immune microenvironment of gynecological mesonephric-like adenocarcinoma (MLA).

Methods

To determine the comprehensive characteristics of MLA, 17 patients with MLA were retrospectively enrolled in this study. Whole-exome sequencing and mRNA sequencing were performed to explore the molecular features. The biological differences between MLAs and epithelial-initiated gynecologic tumors reported in The Cancer Genome Atlas database were also analyzed.

Results

KRAS mutations (82.4%) were considered the driving mechanism and were co-mutated with PIK3CA (47.1%) and SPOP (23.5%), but their functions were mutually exclusive. In addition, pathways and genes associated with kidney development were upregulated i

SUBMITTER: Zeng J 

PROVIDER: S-EPMC12307467 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature