A Validated RP-HPLC Method for Monitoring Pollutants Removal during Microalgae Bioremediation of Polluted Waters.

ABSTRACT: A Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) method was developed and validated to simultaneously quantify six contaminants of emerging concern (CECs) in the context of microalgae bioremediation. RP-HPLC was chosen for its ability to separate substances of differing polarities, its use of cost-effective and low-toxicity mobile phases, and the capacity to selectively adjust pH for enhanced sample resolution. To validate this method, standard guidelines were followed to evaluate system suitability, linearity, accuracy, precision, recovery, limits of detection and quantification, selectivity and specificity. Limits of detection and limits of quantification were, respectively, 0.017 and 0.051 µg mL^-1 for paracetamol (PAR), 0.024 and 0.072 µg mL^-1 for

SUBMITTER: Santos B 

PROVIDER: S-EPMC12307476 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature