Two Centrins and Their Posttranslational Modification Modulate the Cell Cycle of Giardia lamblia.

ABSTRACT: Centrins, Ca²⁺-binding proteins conserved in eukaryotes, are the key components of the microtubule-organizing center. Giardia lamblia possesses two centrins (GL50803_6744: centrin 1; GL50803_104685: centrin 2) localized in the basal bodies during cell division. G. lamblia centrin 2 (Glcent2) is also found in the nuclei of interphase Giardia, with its N-terminal half being necessary for this localization. Morpholino-mediated knockdown of Glcents resulted in abnormal nuclear positioning and cytokinesis, causing cell malformations, including ventral discs and flagella defects. Small ubiquitin-like modifier (SUMO)ylation is a posttranslational modification, which modulates several cellular processes. Here, we demonstrated that Glcents are substrates of SUMO through in vitro SUMOylat

SUBMITTER: Yeo HR 

PROVIDER: S-EPMC12307541 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature