Tumor extracellular matrix enhances invasive gene expression of breast cancer cells in 3D patient-derived scaffolds.

ABSTRACT: Extracellular matrix (ECM) remodeling in cancer provides an essential substructure for tumor progression. We utilized patient-derived scaffolds (PDS) to model tumor ECM changes and study their impact on cell behavior. PDS were obtained from breast tumor and normal healthy breast tissue by decellularizing surgically resected specimens. We used PDS to design a 3D culture of the breast cancer cell line MCF-7. We utilized bioinformatics pipeline to identify hub genes indicative of cell invasiveness, and assessed their expression using quantitative real-time PCR. Our decellularization protocol led to decellularization of tissues while preserving key ECM components. ECM components such as collagen, glycosaminoglycans, collagen IV, and vimentin were significantly overexpressed in tumor compared t

SUBMITTER: Pezeshki PS 

PROVIDER: S-EPMC12307645 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature