Detection of the distribution frequency of Diego blood type based on multiplex droplet digital PCR.

ABSTRACT: The Diego blood group is a crucial blood group system for ensuring the safety of clinical transfusions. However, current detection methods for the Diego blood group remain limited. This study aims to develop a multiplex digital PCR approach for Diego blood group genotyping and establish a blood pool in the northwest region. Additionally, multiplex digital PCR is used to determine the distribution frequencies of the Diego blood group antigens Dia and Dib in this region. The proportions of Di(a+b-), Di(a+b+), and Di(a-b+) phenotypes in a blood pool of 1000 individuals, as determined by multiplex digital PCR, were 0.281%, 6.972%, and 92.747%, respectively. The frequencies of the Dia and Dib antigens in Xi'an region were 7.253% and 99.719%, respectively. This study established a multiplex digi

SUBMITTER: Chu X 

PROVIDER: S-EPMC12307735 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature