Multichannel bioelectronic sensing using engineered Escherichia coli.

ABSTRACT: To advance environmental health and hazard detection, researchers have developed whole-cell bioelectronic sensors by engineering extracellular electron transfer to be dependent on an analyte¹. However, these sensors regulate a single electron transfer pathway as an electrochemical channel, limiting the sensing information to a single analyte. We have developed a multichannel bioelectronic sensor where different chemicals regulate distinct extracellular electron transfer pathways within a single Escherichia coli cell. One channel utilizes the flavin synthesis pathway from Bacillus subtilis² and is controlled by a cadmium-responsive promoter. Another channel, the CymA-Mtr pathway from Shewanella oneidensis³, is controlled by an arsenite-responsive promoter an

SUBMITTER: Zhang X 

PROVIDER: S-EPMC12307765 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature