Advancing in ovo egg sexing through molecular sex identification of chick embryos using LAMP and RPA assays.

ABSTRACT: Despite global efforts, minimizing the culling of one-day hatched male chicks remains a critical priority in the poultry industry due to significant socio-economic concerns. To address this issue, various molecular assays have been developed for in ovo sex determination, enabling the identification and elimination of male embryos at early developmental stages. However, due to their complexity associated with ensuring high precision, the requirement for advanced infrastructures and the time-intensive nature of current methods, these assays have yet to achieve widespread commercialization. In this study, we developed two novel digital readout assays employing PCR, LAMP and RPA techniques, which were evaluated for sensitivity, specificity and robustness using 82 nine-day-old chick embryos. Ou

SUBMITTER: Van Der Hofstadt M 

PROVIDER: S-EPMC12307818 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature