Comparative analysis of 3D-culture techniques for multicellular colorectal tumour spheroids and development of a novel SW48 3D-model.

ABSTRACT: Colorectal cancer (CRC) is an important global health challenge, with nearly 2 million diagnosed cases and over 900,000 deaths annually despite therapeutic advancements. The high morbidity and mortality rates underscore the need for more efficient therapies. Three-dimensional (3D) cell culture models have emerged as more physiologically relevant alternatives to traditional two-dimensional (2D) models for drug screening and mechanistic studies. However, generating consistent spheroids across different CRC cell lines presents technical challenges, and protocols remain inconsistent. This study evaluated different 3D culture methodologies, i.e. overlay on agarose, hanging drop, and U-bottom plates without matrix or with methylcellulose, Matrigel or collagen type I hydrogels, across eight CRC c

SUBMITTER: Mateos-Sanchez C 

PROVIDER: S-EPMC12307937 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature