GPX4 predicts poor prognosis and regulates tumor proliferation and senescence in colorectal adenocarcinoma.

ABSTRACT:

Background

Colorectal adenocarcinoma (COAD) is one of the most common gastrointestinal malignancies. There is a pressing need to recognize reliable biomarkers that can improve diagnostic accuracy, predict prognosis, and serve as effective molecular targets. Glutathione peroxidase 4 (GPX4) is an important antioxidant protein. Evidence demonstrates that abnormal expression of GPX4 is related to cancer initiation and progression. However, the role of GPX4 in COAD remains unclear.

Methods

We employed bioinformatics analysis and conducted subsequent validation of biological processes, including cell counting kit-8 assay (CCK-8), colony formation assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), 5-ethynyl-2'-deoxyuridine assay (EdU), western blot, immu

SUBMITTER: Zhang YU 

PROVIDER: S-EPMC12308247 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature