A Macrophage-Based Cell Therapy Approach Promotes Collagen Deposition in Diabetic Wounds.

ABSTRACT: Macrophages play a critical role in wound healing. Modulating macrophage activity has thus been identified as a potential strategy to stimulate immune-mediated tissue regeneration. This study utilised endotoxin tolerization as a strategy to dampen cytokine production upon the ex vivo activation of M2 monocytic cells before collecting their secretome for therapeutic application. The M2 derived secretome was harvested from GM-CSF differentiated THP-1 cells followed by IL4-induced M2 polarisation with or without prior endotoxin tolerization. The protein constituents of the secretome were determined and quantified using label free LC-MS/MS analysis and cytokine levels using enzyme-linked immunosorbent assays. The efficacy of the M2 derived secretome (with and without prior tolerization) to sti

SUBMITTER: Boodhoo K 

PROVIDER: S-EPMC12308272 | biostudies-literature | 2025 Jul-Aug

REPOSITORIES: biostudies-literature