Structure of the Lipopolysaccharide from : A Chemical Perspective on Immune Recognition.

ABSTRACT: Gram-negative bacterium , which is increasingly prevalent in elderly individuals, is associated with cognitive decline and gut-brain axis dysfunction. Here, we present a comprehensive structural characterization of lipopolysaccharide (LPS), a key modulator of immune recognition and the main component of its outer membrane. Using a multidisciplinary approach combining chemical, spectroscopic, spectrometric, biophysical and computational methods, we unveil a unique O-antigen characterized by a trisaccharide repeating unit containing rhamnose and glucosamine, displaying nonstoichiometric O-acetylation and a terminal methylated rhamnose capping the saccharide chain. Furthermore, we disclose a short core oligosaccharide and a Lipid A composed of penta- to tetra-acylated species. Notably, this L

SUBMITTER: Nieto-Fabregat F 

PROVIDER: S-EPMC12308378 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature