Harnessing the Materials Chemistry of Mesoporous Silicon Nanoparticles to Prepare "Armor-Clad" Enzymes.

ABSTRACT: There is a growing interest in nanomaterials that can encapsulate enzymes while retaining their ability to function within the confines of a nanocage. Here porous silicon nanoparticles (pSiNPs) are evaluated as an enzyme cage, utilizing the aqueous chemistry of silicon to dynamically restructure the mesopore structure, immobilizing, and confining the enzyme. The common bioluminescent reporter enzyme nanoluciferase (Nluc) is used to evaluate two different trapping chemistries, and impacts on the stability and catalytic performance of the enzyme are compared with controls involving free enzyme and enzyme electrostatically adsorbed to a pSiNP host without the use of trapping chemistry. The two chemistries exploited in this study are (1) oxidative trapping, where mild aqueous oxidation of the

SUBMITTER: Lu YS 

PROVIDER: S-EPMC12308502 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature