A New Next-Generation Sequencing Approach in Human Cytomegalovirus for the Identification of Antiviral Resistance Mutations and Genotypic Classification.

ABSTRACT: This study introduces a new procedure for antiviral resistance analysis and genetic classification of human cytomegalovirus (HCMV) using next-generation sequencing (NGS) adapted to existing methodologies, aiming for more targets due to the recent use of new antivirals. It expands the classical investigation of mutations in UL54 and UL97 genes, associated with resistance to (val)ganciclovir, foscarnet and cidofovir, to include UL27, UL56 and UL89 genes, which target newer antivirals like maribavir and letermovir. Additionally, it includes the genetic analysis of UL55 (glycoprotein B) for genotype classification. This new methodology involves multiplex-PCR for DNA enrichment, followed by NGS using Illumina MiSeq platform and data analysis through an in-house pipeline. Several validations wer

SUBMITTER: Arnedo-Munoz M 

PROVIDER: S-EPMC12308776 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature