Effects of matrix metalloproteinase inhibition in cultured human Tenon's capsule fibroblasts.

ABSTRACT:

Background

This study aimed to compare the characteristics of anterior and posterior human Tenon's capsule fibroblasts and determine the effect of matrix metalloproteinase (MMP) inhibition on cultured human Tenon's capsule fibroblasts.

Methods

Tissues obtained from the anterior and posterior Tenon's capsules were partially incised, cultured, and subjected to microarray analysis. Electrophoresis was performed on the cells treated with Ilomastat (MMP-9 inhibitor), and collagen 1, 3, and 6. Antibodies against transforming growth factor-beta (TGF-β) and vimentin were used as probes to compare the expression levels.

Results

There were no differences between anterior and posterior Tenon's capsule fibroblasts in the microarray. Western blot analysis showed similar expression

SUBMITTER: Kim NH 

PROVIDER: S-EPMC12308980 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature