Artificial chromosome reorganization reveals high plasticity of the budding and fission yeast genomes.

ABSTRACT:

Background

The genome of a eukaryotic cell is usually organized on a set of chromosomes. Recently, karyotype engineering has been applied to various organisms, but whether and to what extent a naturally evolved genome can resist or tolerate massive artificial manipulations remains unexplored.

Results

Using unicellular yeast models of both Saccharomyces cerevisiae and Schizosaccharomyces pombe, we deliberately construct dozens of single-chromosome strains with different chromosome architectures. Three S. cerevisiae strains have the individual chromosomes fused into a single chromosome, but with the individual chromosomes in different orders. Eighteen S. cerevisiae strains have a single chromosome but with different centromeric sequences. Fifteen S. cerevisiae strains have a s

SUBMITTER: Zhu X 

PROVIDER: S-EPMC12309097 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature