Engineering Streptomyces coelicolor for heterologous expression of the thiopeptide GE2270A-A cautionary tale.

ABSTRACT: The thiopeptide GE2270A is a clinically relevant, ribosomally synthesised, and post-translationally modified peptide naturally produced by Planobispora rosea. Due to the genetically intractable nature of P. rosea, heterologous expression is considered a possible route to yield improvement. In this study, we focused on improving GE2270A production through heterologous expression of the biosynthetic gene cluster (BGC) in the model organism Streptomyces coelicolor M1146. A statistically significant yield improvement was obtained in the S. coelicolor system through the data-driven rational engineering of the BGC, including the introduction of additional copies of key biosynthetic and regulatory genes. However, despite our best efforts, the highest production level observed in the strains gener

SUBMITTER: Del Carratore F 

PROVIDER: S-EPMC12309360 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature