A rapid and naked-eye methicillin resistant <i>Staphylococcus aureus</i> screening method based on CRISPR/Cas12a and hybridization chain reaction.

ABSTRACT: Methicillin-resistant Staphylococcus aureus (MRSA), a widely drug-resistant bacterium, poses a significant threat to global health. Current culturing and nucleic acid detection methods are time-consuming and require complex instruments, which do not meet the detection needs. Herein, we developed a rapid and visual MRSA detection method (MCFHCR) using ssDNA-functionalized magnetic beads as a trigger chain combined with the trans-cleavage activity of the Cas12a protein and fluorescence signal amplification of the hybridization chain reaction (HCR). MCFHCR is a signal-off platform for the detection of MRSA. In the absence of DNA targets, the trans-cleavage activity of Cas12a is inactivated, allowing HCR to proceed and form long double-stranded DNA, resulting in an increased fluorescent

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC12309411 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature