Generation of an Inducible Destabilized-Domain Cre Mouse Line to Target Disease Associated Microglia.

ABSTRACT: The function of microglia during progression of Alzheimer's disease (AD) can be investigated using mouse models that enable genetic manipulation of microglial subpopulations in a temporal manner. We developed mouse lines that express either Cre recombinase (Cre) for constitutive targeting, or destabilized-domain Cre recombinase (DD-Cre) for inducible targeting from the Cst7 locus (Cst7 ^DD-Cre) to specifically manipulate disease associated microglia (DAM) and crossed with Ai14 tdTomato cre-reporter line mice. Cst7^Cre was found to target all brain resident myeloid cells, due to transient developmental expression of Cst7, but no expression was found in the inducible Cst7 ^DD-Cre mice. Further crossing of this line with 5xFAD mice combined with dietary administr

SUBMITTER: Henningfield CM 

PROVIDER: S-EPMC12309518 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature