A kinetic model of copper homeostasis in Saccharomyces cerevisiae.

ABSTRACT: Rather than inhibiting copper entry when grown on high Cu, yeast cells import excessive Cu while simultaneously increasing expression of metallothionein CUP1 which then sequesters excess Cu. An ordinary-differential-equations-based kinetic model was developed to investigate this unusual behavior. The assumed reaction network included 25 reactions and 10 components in the cytosol of yeast cells growing in media supplemented with a series of increasing nutrient COPPER concentrations. Published concentrations of copper proteins and coordination complexes that constitutes the low-molecular-mass (or labile) Cu pool were assumed. Other components included transcription factors MAC1 and ACE1, the MAC1-dependent copper importer CTR1, and other copper proteins considered collectively. A second MAC1

SUBMITTER: Dulaney C 

PROVIDER: S-EPMC12309601 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature