Protocol for generation, quantification, and phenotyping of brain metastases in preclinical mouse models.

ABSTRACT: The field of brain metastasis is rapidly expanding, yet no consensus exists on the most reliable quantification approach. We present a protocol for assessing metastatic burden in mice following intracarotid injection of tumor cells. We describe steps for surgical procedures, brain processing, cryo-sectioning, and slide preparation, followed by phenotypic characterization. We detail procedures for quantifying brain metastatic area using automated microscopy and semi-supervised image analysis. For complete details on the use and execution of this protocol, please refer to Rodriguez-Baena et al.¹.

SUBMITTER: Rodriguez-Baena FJ 

PROVIDER: S-EPMC12309607 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature