Successful selection of cross-protective vaccine candidates for Ornithobacterium rhinotracheale infection.

ABSTRACT: Ornithobacterium rhinotracheale is a bacterial pathogen known for causing respiratory disease in poultry. In this study, we demonstrate for the first time that cross-protective immunity against different O. rhinotracheale serotypes can be induced by live vaccination. Sera from these live-vaccinated and cross-protected birds were used to identify new vaccine targets by screening an O. rhinotracheale expression library. Out of 20,000 screened plaques, a total of 30 cross-reactive clones were selected for further analysis. Western blot analysis and DNA sequencing identified eight different open reading frames. The genes encoding the eight cross-reactive antigens were amplified, cloned in an expression vector, and expressed in Escherichia coli. Purified recombinant proteins with a molecular ma

SUBMITTER: Schuijffel DF 

PROVIDER: S-EPMC1230975 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature