Shaping and interpretation of Dpp morphogen gradient by endocytic trafficking.

ABSTRACT: Dpp/BMP is a morphogen that controls the patterning and growth of the Drosophila wing disc. Although endocytic trafficking has been proposed to influence both extracellular Dpp distribution and signaling, how this process shapes and interprets the Dpp gradient under physiological conditions remains unclear due to limitations in visualizing endogenous Dpp. Here, we generated fluorescent protein-tagged functional dpp alleles that allow simultaneous visualization of extracellular and intracellular Dpp distributions. Using these tools, we found that, while Dynamin-mediated internalization is required for Dpp signaling activation, Rab5-mediated early endosomal trafficking is dispensable for Dpp spreading and signaling initiation but is required for signal termination by promoting the downregula

SUBMITTER: Rasouliha SH 

PROVIDER: S-EPMC12310002 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature