Late-stage labeling of diverse peptides and proteins with iodine-125.

ABSTRACT: The preparation of specifically iodine-125 (¹²⁵I)-labeled peptides of high purity and specific activity represents a key tool for the detailed characterization of their binding properties in interaction with their binding partners. Early synthetic methods for the incorporation of iodine faced challenges such as harsh reaction conditions, the use of strong oxidants and low reproducibility. Herein, we review well-established radiolabeling strategies available to incorporate radionuclide into a protein of interest, and our long-term experience with a mild, simple and generally applicable technique of ¹²⁵I late-stage-labeling of biomolecules using the Pierce iodination reagent for the direct solid-phase oxidation of radioactive iodide. General recommendations, tips, and d

SUBMITTER: Marek A 

PROVIDER: S-EPMC12310069 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature