Dissecting rifampicin heteroresistance in <i>Mycobacterium tuberculosis</i>: integrating whole-genome sequencing with phenotypic and clonal validation.

ABSTRACT: Introduction. This study underscores the critical role of identifying heteroresistant infections of Mycobacterium tuberculosis (Mtb) in enhancing the diagnostics of tuberculosis (TB). These conditions complicate diagnostics and treatment, underlining the need for advanced techniques to detect and characterize resistant populations effectively.Hypothesis/Gap statement. Current diagnostics may fail to identify heteroresistance and mixed infections, limiting the understanding of their impact on treatment outcomes.Aim. This pilot study aimed to phenotypically and genotypically characterize rifampicin-heteroresistant clinical isolates and assess their genetic diversity and resistance patterns.Methodology. A retrospective analysis of 2,917 Mtb genomes from Peru

SUBMITTER: Vallejos-Sanchez K 

PROVIDER: S-EPMC12310241 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature