The impact of thyroid hormone concentration fluctuations on colon cancer proliferation and growth.

ABSTRACT:

Background

To examine the effects of thyroid hormone (TH) levels on colon cancer progression, HCT-116 colon cancer cells were inoculated into the axillary region of thymus-deficient male BALB/c nude mice. Mild hypothyroidism and hyperthyroidism were then induced to observe tumor growth patterns under different TH conditions.

Methods

Following subcutaneous tumor implantation, mice were randomly divided into three groups: hyperthyroid (levothyroxine-treated), hypothyroid (methimazole-treated), and control (saline-treated). Tumor volume and final mass were monitored throughout the study period. Excised tumors were subjected to histological analysis including hematoxylin-eosin (HE) staining, immunofluorescence, and TUNEL assay for apoptosis detection.

Results

In the tumor

SUBMITTER: Ren F 

PROVIDER: S-EPMC12310445 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature