Dental pulp stem cells conditioned medium-functionalized microspheres for endodontic regeneration.

ABSTRACT:

Introduction

Dental pulp disease represents a prevalent oral pathology with limited success in functional regeneration of the pulp-dentin complex. Stem cell-based endodontic regenerative therapy has emerged as a promising approach, leveraging the principles of tissue engineering through the combination of stem cells, scaffolds, and growth factors. However, recreating the native pulp microenvironment remains a critical challenge.

Methods

This study developed a novel strategy to mimic the dental pulp regeneration microenvironment using nucleus pulposus microspheres (NPM) loaded with conditioned medium (CM) for dental pulp stem cells (DPSCs). The biocompatibility of NPM and the effects of NPM-loaded CM on DPSCs differentiation and angiogenesis were systematically evaluated. A s

SUBMITTER: Gao J 

PROVIDER: S-EPMC12310587 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature