Keratinocyte-derived VEGF-A is an essential pro-migratory autocrine mediator, acting through the KDR/GEF-H1/RhoA pathway.

ABSTRACT:

Introduction

Keratinocytes proliferate, migrate and differentiate to achieve skin re-epithelialization following injury. They also secrete soluble mediators to induce inflammation and orchestrate restoration of the skin barrier. However, dysregulated mediator release can cause sustained inflammation, leading to pathological healing. The small GTPase RhoA is key for cell migration, but the molecular mechanisms controlling Rho proteins in keratinocytes remain incompletely characterized. The overall objective of the current study was to explore the connection between inflammation-induced keratinocyte mediator release and enhanced migration, and to identify specific RhoA regulators involved.

Methods

The study was done using HaCat cells and primary adult keratinocytes. A multiple

SUBMITTER: Maksimoska V 

PROVIDER: S-EPMC12310649 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature