Nanopore sequencing and hybrid assembly: unraveling the genomic landscape of dollar spot with enhanced annotation and drug resistance profiling.

ABSTRACT: The increasing multi-drug resistance observed in the turfgrass pathogen Clarireedia spp. has emerged as a critical issue. Understanding the mechanisms underlying fungicide resistance is crucial to address this challenge. This study focuses on comparing a highly propiconazole-resistant isolate of Clarireedia jacksonii, HRI11, with a sensitive isolate, HRS10. Genomes were sequenced using the Oxford Nanopore MinION sequencing platform, and hybrid assembly was performed using this data and existing Pacific Biosciences long reads and Illumina short reads. HRI11 genome assembly represents the most contiguous and complete genome assembly reported for Clarireedia to date, spanning 43.6 MB with 12,831 predicted protein-coding genes across 51 scaffolds. In contrast, the HRS10 ha

SUBMITTER: Shi X 

PROVIDER: S-EPMC12310695 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature