Thymol and menthol as anaesthetics for short transportation of zebrafish larva.

ABSTRACT: Transportation-induced stress is a major concern in aquaculture, often causing physiological and behavioural disruptions in fish. To alleviate these effects, anaesthesia is commonly used, but the most widely used anaesthetics, MS-222 and eugenol, have been linked to adverse effects, and alternatives are lacking. This study evaluated the anaesthetic efficacy of thymol and menthol, in comparison with MS-222 and eugenol, under stress conditions induced by a simulated 4-h transportation of 96 hpf zebrafish larvae. Based on LC₅₀ values, the transport simulation was conducted with 200 mg L^-1 MS-222, 80 mg L^-1 eugenol, 15 mg L^-1 thymol, and 50 mg L^-1 menthol. Parameters assessed included heart rate, biochemical markers, stress responses, meta

SUBMITTER: Vieira RSF 

PROVIDER: S-EPMC12310772 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature