Resolving floral development dynamics using genome and single-cell temporal transcriptome of Dendrobium devonianum.

ABSTRACT: Dendrobium devonianum, a species of the Orchidaceae family, is notable for its unique floral characteristics, which include two yellow spots and purple tips on its labellum, as well as fringed edges. However, the molecular mechanisms underlying flower pattern formation in D. devonianum remain poorly understood, hindering advancements in its breeding process. Here, a chromosome-scale genome of D. devonianum was presented for the first time, revealing two significant polyploidization events. Additionally, a high-resolution single-cell transcriptomic atlas was constructed, capturing 11 distinct cell clusters. Expression patterns of MADS-box genes were identified through temporal and spatial bulk RNA-Seq, revealing alignment with the ABCDE model of flower formation. Meanwhile, mass spectrometr

SUBMITTER: Wang J 

PROVIDER: S-EPMC12310821 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature