Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila.

ABSTRACT: Filamentous fungi produce large quantities of cellulase and xylanase as extracellular enzymes to degrade plant-derived polysaccharides. This process is controlled by a complex network of transcription factors (TFs). Here, we present the bZIP TF Mthac-1 exhibiting dual regulatory effects on the production of cellulase and xylanase in Myceliophthora thermophila. The deletion of Mthac-1 reduced the cellulase and xylanase activities and protein secretion during the early phase of cultivation but enhanced in the middle and late stages of cultivation, compared with the wild-type (WT) strain. It also led to fungal growth defects, characterised by few hyphal branching and reduced conidiation. Real-time quantitative reverse transcription PCR (RT-qPCR) analysis showed that Mthac-1 dynamically regula

SUBMITTER: Lai Y 

PROVIDER: S-EPMC12310822 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature