Replacement of large subunit N terminus enabled biogenesis of different plant Rubiscos in E. coli.

ABSTRACT: The efforts of engineering plant ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) with the goal of improving plant photosynthetic efficiency and crop yield have existed for long. However, the directed evolution of plant Rubisco has not been widely explored because its biogenesis in a heterologous host such as Escherichia coli remains challenging. Recent breakthroughs in developing the Arabidopsis five-auxiliary-chaperone package and optimizing the chaperone origins have enabled the functional assembly of several plant Rubisco large subunits with their native or other plant small subunits in E. coli. But tedious and unpredictable optimization of chaperone origins might still be required for the assembly of another plant Rubisco. Here, we identified several residues at the N terminu

SUBMITTER: Cai Z 

PROVIDER: S-EPMC12310878 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature