Optimizing plant size for vertical farming by editing stem length regulators.

ABSTRACT: Vertical farming offers the advantage of providing a stable environment for plant cultivation, shielding them from adverse conditions such as climate change. For fruit-harvesting plants like tomato, vertical farming necessitates the optimization of plant growth and architecture. The gibberellin 3-oxidase (GA3ox) genes encode gibberellin 3-oxidases responsible for activating GA within the pathway and modulating stem length. Among the five SlGA3ox genes, we targeted the coding regions of three SlGA3ox genes (named SlGA3ox3, SlGA3ox4 and SlGA3ox5) using multiplex CRISPR genome editing. The slga3ox4 single mutants exhibited a slight reduction in primary shoot length, leading to a smaller stature. In contrast, the slga3ox3 and slga3ox5 single mutants showed subtle phenotypic changes. Notably, t

SUBMITTER: Lim Y 

PROVIDER: S-EPMC12310881 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature