A highly sensitive method for detecting African swine fever virus in oral fluids from naturally infected pigs in Northern Vietnam.

ABSTRACT: Early detection and early slaughter through quarantine are essential to prevent the spread of the African swine fever virus (ASFV). Pooled oral fluids testing is a practical approach for pathogen monitoring, but compared to blood, the virus concentration is lower, increasing false negatives. We developed a highly sensitive method for ASFV detection in pig oral fluids using a pretreatment-based concentration protocol. We collected oral fluids from sub-clinical raised pigs in northern Vietnam and conducted a field evaluation using the developed method. A spike test showed up to 100-fold greater sensitivity than a reference method. For performance evaluation, 68 pooled oral fluid samples were collected, of which 63 originated from raised pigs in northern Vietnam and 5 were obtained from healt

SUBMITTER: Mai TN 

PROVIDER: S-EPMC12311203 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature